Using a Xenogeneic Acellular Dermal Matrix Membrane to Enhance the Reparability of Bone Marrow Mesenchymal Stem Cells for Cartilage Injury

Due to its avascular organization and low mitotic ability, articular cartilage possesses limited intrinsic regenerative capabilities. The aim of this study is achieve one-step repair in situ via combining bone marrow stem cells (BMSCs) with a xenogeneic Acellular dermal matrix (ADM) membrane. ADM membranes were harvested from Sprague-Dawley (SD) rats through standard decellularization procedures. characterization the scaffolds was measured, including morphology physical properties vitro experiments included cell distribution, chondrogenic quantification, viability evaluation scaffolds. Adult male New Zealand white rabbits used for vivo evaluation. Isolated microfracture performed control (MF group) left knee tested group as an experimental when scaffold implanted matching defect after right knee. At 6, 12, 24 weeks post-surgery, sacrificed further research. could adsorb water had excellent porosity. grew well seeded on scaffold, demonstrating characteristic spindle-shaped morphology. exhibited proliferative capacity cartilaginous collagen production BMSCs. In rabbit model, showed earlier filling, more hyaline-like neo-tissue formation, better interfacial integration between defects normal compared (MF) at post-surgery. addition, neither intra-articular inflammation nor rejection reaction observed implantation scaffold. This provides promising biomaterial-based strategy worth investigation large animal models.